Fig. 5

Piperine treatment ameliorated Purkinje cell degeneration in TBP-105Q KI mice. a Western blotting analysis of calbindin levels in the cerebellum of TBP-105Q KI mice treated with saline (TBP + NS) or piperine (TBP + Pip). Actin was used as a loading control. b Quantification of the ratios of calbindin to actin on western blots in Fig. 5a (n = 3, * P < 0.05). c Immunohistochemistry staining of calbindin in the cerebellum of TBP-105Q KI mice treated with saline or piperine (Scale bar: left, 50 μm; right, 20 μm). d Western blotting analysis of mutant TBP in the cerebellum of TBP KI mice treated with saline or piperine. Four mice per group were examined. Aggregated and soluble forms of TBP are indicated. e Quantification of western blotting result in Fig. 5d (n = 4). Results are presented as means ± SEM