Fig. 3
From: Pathological phosphorylation of tau and TDP-43 by TTBK1 and TTBK2 drives neurodegeneration
Expression of hTTBK1-cat causes tau-dependent neuron loss and axonal abnormalities. GFP-labeled D-type GABAergic motor neurons were observed in vivo in live day 1 adult transgenic C. elegans. a Fluorescent images of GABAergic live neurons in the posterior region. Each live neuron is marked with an asterisk. b Fluorescent images of GABAergic dorsal cord. Gaps in dorsal cord are marked with brackets (c) Fluorescent images of GABAergic axonal commissures. Aberrantly branched commissures are marked with arrows. d Number of neurons lost for each worm is plotted. GFP-labeled controls lost an average of 0.22 neurons per animal (n = 22). hTTBK1-cat Tg animals lost an average of 0.06 neurons (n = 46). Tau Tg animals lost an average of 2.4 neurons (n = 56). hTTBK1-cat;tau Tg animals lost an average of 3.8 Neurons (n = 49). P < 0.001 for tau versus hTTBK1-cat;tau. e Number of dorsal cord gaps for each worm is plotted. hTTBK1-cat Tg animals had an average of 0.14 gaps (n = 21). Tau Tg animals had an average of 2.6 gaps (n = 18). hTTBK1-cat;tau Tg animals had an average of 7.4 gaps (n = 10). P < 0.001 for tau versus hTTBK1-cat;tau (f) Number of aberrantly branched commissures for each worm is plotted. hTTBK1-cat Tg animals had an average of 0 aberrantly branched neurons (n = 20). Tau Tg animals had an average of 1.9 aberrantly branched neurons (n = 18). hTTBK1-cat;tau Tg animals had an average of 5.9 aberrantly branched neurons (n = 20). P < 0.001 for tau versus TTBK1-cat;tau. Significance was determined using a one-way analysis of variance with Tukey’s multiple comparison test among strains. Scale bar = 50 μm