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Fig. 4 | Molecular Neurodegeneration

Fig. 4

From: Pathological phosphorylation of tau and TDP-43 by TTBK1 and TTBK2 drives neurodegeneration

Fig. 4

Expression of hTTBK2-cat causes tau-dependent neuron loss and axonal abnormalities. GFP-labeled D-type GABAergic motor neurons were observed in day 1 adult transgenic animals in vivo. a Fluorescent images of GABAergic live neurons in the posterior region. Each live neuron is marked with an asterisk. b Fluorescent images of GABAergic dorsal cord. Gaps in dorsal cord are marked with brackets (c) Fluorescent images of GABAergic axonal commissures. Aberrantly branched commissures are marked with arrows. d Number of neurons lost for each worm is plotted. hTTBK2-cat Tg animals lost an average of 0.13 neurons (n = 23). Tau Tg animals lost an average of 1.4 neurons (n = 29). hTTBK2-cat;tau Tg animals lost an average of 2.9 Neurons (n = 23). P < 0.001 for tau versus hTTBK2-cat;tau (e) Number of dorsal cord gaps for each worm is plotted. hTTBK2-cat Tg animals had an average of 0.27 gaps (n = 22). Tau Tg animals had an average of 1.1 gaps (n = 28). hTTBK2-cat;tau Tg animals had an average of 2.3 gaps (n = 20). P < 0.001 for tau versus hTTBK2-cat;tau (f) Number of aberrantly branched commissures for each worm is plotted. hTTBK2-cat Tg animals had an average of 0.09 aberrantly branched neurons (n = 23). Tau Tg animals had an average of 0.9 aberrantly branched neurons (n = 29). hTTBK2-cat;tau Tg animals had an average of 4.1 aberrantly branched neurons (n = 21). P < 0.001 for tau versus hTTBK2-cat;tau. Significance was determined using a one-way analysis of variance with Tukey’s multiple comparison test among strains. Scale bar = 50 μm

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