Fig. 7
From: Pathological phosphorylation of tau and TDP-43 by TTBK1 and TTBK2 drives neurodegeneration
FTLD cases exhibit increased TTBK1 immunostaining. Representative images demonstrating upregulation of TTBK1 immunoreactivity in FTLD brain tissue (n = 5 cases of FTLD-TDP represented in panels b,e; n = 7 cases of FTLD-tau represented in panels (c, f)) compared to that of normal control subjects (a, d). In the hippocampus (d-f), the increase in TTBK1 is highly robust in FTLD-tau cases relative to both FTLD-TDP cases and controls. In the frontal cortex (a-c) there is enhanced TTBK1 immunoreactivity relative to normal controls, but the difference between the FTLD subtypes was less apparent. Scale bar = 50 μm. g TTBK1 is expressed throughout the cytoplasm, and overlaps with phosphorylated tau (AT180) in human cells. Shown is an image from a representative case (n = 3). Pearson coefficient of correlation for colocalization = 0.69 ± 0.18 for n = 46 neurons analyzed