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Fig. 5 | Molecular Neurodegeneration

Fig. 5

From: BACE1 elevation engendered by GGA3 deletion increases β-amyloid pathology in association with APP elevation and decreased CHL1 processing in 5XFAD mice

Fig. 5

GGA3 deletion prevents age-dependent elevation of BACE1 in 5XFAD mice. a Representative immunoblots of hippocampus homogenates from 2, 4, 7 and 12 months old GGA3WT;5XFAD, GGA3Het;5XFAD and GGA3KO;5XFAD mice probed with anti-BACE1 (D10E5), and anti-GAPDH (MAB374) antibodies. b BACE1 levels normalized to GAPDH in hippocampus from 5XFAD mice at 2, 4, 7, and 12 months of age are presented relative to the levels of BACE1 in homogenates from 2 months old GGA3WT;5XFAD mice (set to 1.0). 12 months old GGA3WT;5XFAD mice have significantly higher BACE1 levels than 2 months old GGA3WT;5XFAD mice. However, age-dependent elevation of BACE1 was not observed in 12 months old GGA3KO;5XFAD mice compared to 2 months old GGA3KO;5XFAD mice. c The table shows statistical analysis of BACE1 levels in hippocampus of GGA3WT;5XFAD, GGA3Het;5XFAD and GGA3KO;5XFAD mice within the same age group. Total number of mice in each group is indicated within bars. All graphs represent mean ± SEM. Two-way ANOVA with Fisher’s LSD post hoc tests was applied to each genotype group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

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Molecular Neurodegeneration

ISSN: 1750-1326