Fig. 1

Phosphorylation of Rab GTPases by LRRK2. a Representative phosphoimages and commassie brilliant blue (CBB)-stained gel images from the in vitro LRRK2 kinase assays with forty-five Rab GTPases. LRRK2 autophosphorylation and Rab phosphorylation are indicated by blue and red arrowhead, respectively. b The level of Rab phosphorylation was normalized against both the protein level of each Rab from CBB staining and the level of LRRK2 autophosphorylation. Extent of phosphorylation is color-coded: potent phosphorylation in red and marginal in green. Data are mean ± SD (n = 3). c Representative phosphoimages and CBB-stained gel images from LRRK2 in vitro kinase assays with Rab1a, 3c, 8a, and 35