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Fig. 2 | Molecular Neurodegeneration

Fig. 2

From: Dysregulated phosphorylation of Rab GTPases by LRRK2 induces neurodegeneration

Fig. 2

Identification of Rab phosphorylation site. a (Top) Tandem mass spectrometry (MS/MS) spectrum of Rab8a phosphorylated by LRRK2 indicates T72 as a phosphorylation site. (Bottom) Underlined text indicates sequence coverage by MS/MS. Phosphorylation site is indicated in red with an asterisk. b Local amino acid sequence alignment surrounding the LRRK2 phosphorylation site in Rab GTPases. Phosphorylation site is indicated with an asterisk. Amino acids are color-coded: aliphatic amino acids in blue, polar in red, acidic in green, basic in purple, and aromatic in yellow. c Validation of phosphorylation site identified by MS/MS. Representative phosphoimages and CBB-stained gel images from LRRK2 in vitro kinase assays performed in the presence of phosphodeficient or phosphomimetic mutant of each Rab (Rab1a T75A or T75D; Rab3c T94A or T94D, Rab8a T72A or T72D; Rab35 T72A or T72D)

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