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Fig. 5 | Molecular Neurodegeneration

Fig. 5

From: Dysregulated phosphorylation of Rab GTPases by LRRK2 induces neurodegeneration

Fig. 5

Phosphomutants of Rab induce neurotoxicity in primary cortical neurons. a, b Embryonic day 15.5 cortical neurons were transfected with wild-type (WT) or mutants of myc-tagged LRRK2 or GFP-conjugated Rab (Rab1a, 3c, 8a, or 35) at DIV 7. Neurons were fixed at the indicated time points after transfection and immunostained with either anti-myc (for cells transfected with LRRK2 constructs) or anti-GFP (for cells transfected with Rab constructs) antibodies to identify transfected cells. Representative images (a) and quantification of neurite length (b) are shown. Data represent mean ± SD from three (a) or five (b) independent experiments, and in any one experiment, at least 20 neurons per construct were analyzed

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