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Fig. 6 | Molecular Neurodegeneration

Fig. 6

From: Dysregulated phosphorylation of Rab GTPases by LRRK2 induces neurodegeneration

Fig. 6

Neurotoxicity induced by LRRK2 substrate Rab GTPases in primary cortical neurons. a, b Embryonic day 15.5 cortical neurons were transfected at DIV 7 as in Fig. 5 and fixed at 2 (a) or 7 days (b) after transfection. Neurons were immunostained with either anti-myc (for cells transfected with LRRK2 constructs) or anti-GFP (for cells transfected with Rab constructs) antibodies to identify transfected cells. Neurons were also immunostained for TUNEL, and TUNEL-positive neurons were counted and presented as percentage of total neurons expressing the construct of interest. Data are mean ± SD from six independent experiments, and in any one experiment, at least 40 neurons per construct were analyzed. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001; one-way ANOVA followed by Dunnett’s multiple comparison post hoc test

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