Fig. 2From: Genetic ablation of dynactin p150Glued in postnatal neurons causes preferential degeneration of spinal motor neurons in aged miceDynein/dynactin complex remains intact in Dctn1LoxP/LoxP; Cre/Esr1 neurons. a-b Sucrose density gradient centrifugation shows p150Glued and p135+ predominantly migrate at fraction 4 and 5 in a 5–20% sucrose gradient similar to the other dynactin subunits p62, p50, and ARP1, as well as the dynactin-interacting dynein subunit DIC in the control Dctn1LoxP/LoxP (a) and p150Glued cKO Dctn1LoxP/LoxP; Cre/Esr1 cortical neuron cultures (b) at 7 DIVBack to article page