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Fig. 8 | Molecular Neurodegeneration

Fig. 8

From: Genetic ablation of dynactin p150Glued in postnatal neurons causes preferential degeneration of spinal motor neurons in aged mice

Fig. 8

P150Glued deficiency alters autophagosome and lysosome marker protein expression in spinal motor neurons. a Western blot analysis shows the expression of ATG3, ATG5, ATG7, LC3, LAMP1 and LAMP2 in the spinal cord homogenate of 9-month-old Dctn1LoxP/LoxP and Dctn1LoxP/LoxP; Thy1-Cre mice. Actin was used as loading control. b Bar graphs show the quantification of ATG3, ATG5, ATG7, LC3, LAMP1 and LAMP2 level normalized with actin (n = 10 per genotype). Data were presented as mean ± SEM; **p < 0.01, ***p < 0.001. Unpaired t-test was used for statistical analysis. c Representative images show LAMP2 (green), CHAT (red) and p150Glued (purple) staining in the lumbar spinal cord of 9-month-old Dctn1LoxP/LoxP and Dctn1LoxP/LoxP; Thy1-Cre mice. Four mice per genotype and 10 sections per mouse were examined. Scale bar: 20 μm

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Molecular Neurodegeneration

ISSN: 1750-1326