Fig. 4

Subretinal neovascularization in cone^ΔVhl mice. a-c Immunostaining of cross-sections of the central (a) and peripheral (b) retina of 4-week-old cone^ΔVhl and age-matched ctrl mice (c, central retina) with isolectin. Arrowheads indicate vessels extending from the deep plexus into the ONL in central retinas of cone^ΔVhl mice. DP: deep plexus, IP: intermediate plexus, PP: primary plexus. Scale bar 50 μm. d, e Light microscopy of 4-week-old cone^ΔVhl and ctrl mice. Neovessels (arrowheads) reached the RPE, but did not cross Bruch’s membrane. Scale bar 20 μm. f, g Fundus imaging and fluorescein angiography of cone^ΔVhl mice and ctrl mice at 4 weeks of age. Signs of leakage (arrowhead) were detected in cone^ΔVhl mice. h-k Retinal cross-sections of ctrl h, j and cone^ΔVhl mice (i, k) were stained with antibodies against ALB (h, i) and IBA1 (j, k) at 4 weeks of age. Microglia/macrophages were found within the photoreceptor layer and RPE in cone^ΔVhl mice (arrowheads). Scale bar 50 μm. Abbreviations as in Fig. 1