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Fig. 6 | Molecular Neurodegeneration

Fig. 6

From: Targeting Hif1a rescues cone degeneration and prevents subretinal neovascularization in a model of chronic hypoxia

Fig. 6

Combined deletion of Hif1a and Vhl (cone∆VhlHif1a) rescues the defects in retinal function, morphology and vasculature observed in cone∆Vhl mice. a, b Average single-flash photopic ERGs and b-wave amplitudes showed no significant differences in cone∆VhlHif1a mice (green) compared to ctrl mice (R91W;Nrl-/-;Vhlf/f; Hif1af/f, black) at 12 weeks of age. n=4 per group. b-wave amplitudes are shown as means ± SD. c, d Light microscopy analysis and measurement of the ONL thickness in cone∆VhlHif1a (green), ctrl mice (R91W;Nrl-/-;Vhlf/f; Hif1af/f, black) and coneΔVhl mice (orange, as shown in Fig. 3b) at indicated time points. Abbreviations as in Fig. 1. Scale bar 50 μm. e 3D-reconstruction of blood vessels stained with isolectin on retinal flat mounts of ctrl (left), coneΔVhl (middle) and cone∆VhlHif1a mice (right). Inactivation of Hif1a (cone∆VhlHif1a) rescued the pathological neovascularization. Analysis at 4 weeks of age, n=3. For better recognition and distinction the z-value of the z-stacks was increased five times. The yellow line indicates the border between the outer plexiform layer (OPL) and the ONL. DP: deep plexus, IP: intermediate plexus, PP: primary plexus. f Relative gene expression of hypoxic target genes in cone∆VhlHif1a mice (green) and ctrl mice (R91W;Nrl-/-;Vhlf/f; Hif1af/f, black) at 4we, 8we and 12we as indicated. Abbreviations as in Figs. 1 and 3. Expression levels were normalized to Actb and compared to 4-week-old ctrl mice, which were set to 1. Shown are means ± SD. n≥3 per time point. Scale bar 50 μm

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