Fig. 3

Levels of tau oligomers are specifically reduced in A53T mice treated with TOMA. a-c Western blot of PBS soluble total brain homogenate with tau oligomer-specific antibody, T22, showed that levels of tau dimer (low molecular weight oligomers) were significantly lowered in TOMA-treated mice compared to control IgG-treated mice (A; p = 0.009), as well as 250 kD (high molecular weight) tau oligomers (B; p = 0.036). d-f Tau monomer levels measured by Western blot with Tau5 were unaffected by treatment, while tau dimer was significantly decreased with TOMA. LE (low exposure) HE (high exposure). *Denotes non-specific band. f-g Western blot of the PBS soluble fraction of whole brain homogenate with T22 for tau oligomers showed that TOMA-treated mice had significantly lower levels of a 37 kD fragment compared to Control IgG (p = 0.003). h-m Immunofluorescence imaging detects significantly lower levels of tau oligomers in cortex (h-j, n) and hippocampi (k-m, o) of TOMA-treated mice. Control IgG-treated mice had significantly higher levels of T22 fluorescence intensity as a ratio to nuclei when compared to wildtype mice (p = 0.004) and TOMA-treated A53T mice (p = 0.009) in the cortex, as well as significantly higher levels in the hippocampus compared to wildtype mice (p = 0.001) and TOMA-treated A53T mice (p = 0.0044). Scale bar 50 μm