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Fig. 4 | Molecular Neurodegeneration

Fig. 4

From: Amyloid-beta modulates microglial responses by binding to the triggering receptor expressed on myeloid cells 2 (TREM2)

Fig. 4

The positively charged amino acids in TREM2 mediate its interaction with oAβ1–42. a Schematic representation of mutagenesis of positively charged amino acids on sTREM2-Fc. b Immunoblotting of sTREM2-Fc mutants in the conditioned medium or cell lysate from HEK 293 T cells. (c and d) Bar graphs showing the quantification of Western blots from panel B (n = 3, one-way ANOVA). ND: not determined. e The purified Fc and sTREM2-Fc mutants were analyzed by silver staining. f Solid phase binding assay showing the binding affinity of oAβ1–42 to sTREM2-Fc mutants (n = 3, two-way ANOVA). Data information: Data represent mean ± SD. ***, p < 0.001

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Molecular Neurodegeneration

ISSN: 1750-1326