Fig. 5
Oligomeric Aβ1–42 modulates microglial responses dependent on TREM2 receptor. a Primary microglial cells (5 × 104) from WT or Trem2-knockout (KO) mice were plated onto transwell chamber inserts. Following 20 h incubation with vehicle, oAβ1–42 (0.5 μM), or 10% FBS, cells migrated through the membrane were stained with hematoxylin and eosin and imaged under Nikon inverted microscope. Scale bar, 100 μm. b At least 24 different fields from three independent experiments of transwell tests were selected for quantifying the number of migrated cells (two-way ANOVA). c WT or Trem2-KO (KO) mice brain was harvested after FAM-oAβ1–42 injection for 4 h. Coronal sections from these mice were stained with DAPI (blue) for nuclei, and Iba1 (red) for microglia. Representative z stack images of oAβ1–42-bearing regions are shown. Scale bar, 50 μm. d At least 16 fields (212 μm × 100 μm, marked by white dotted lines in the panel C) from four mice were selected for quantifying the number of microglia (co-stained with DAPI and Iba1) clustered in the oAβ1–42-bearing regions (unpaired, Student’s t-test). e The Iba1-positive area within the white dotted lines in the panel C was normalized to that of FAM-oAβ1–42-positive area (unpaired, Student’s t-test). Data information: Data represent mean ± SD. **, p < 0.01; ***, p < 0.001; ns, not significant