Fig. 3
From: Apolipoprotein E4 impairs spontaneous blood brain barrier repair following traumatic brain injury
Characterization of the NVU response in wildtype mice following TBI. (a) Representative image of toluidine blue stained microvessels isolated from the ipsilateral cortex. RT-QPCR analysis shows the purity of microvessel preparations, consisting of enriched endothelial (Glut1) and pericyte (PDGFRβ) markers with minimal astrocytic expression (GFAP/S100β) in comparison to whole cortex punch fractions. Wildtype mice were subject to TBI before microvessels isolated at 1, 3 and 7 days post-injury and subject to RNA analysis. RT-QPCR analysis of the mRNA expression for (b) apoe (n = 3, *p < 0.05), (c) Mmp-9 (n = 5–6, ***p < 0.001), (d) Pdgfrβ (n = 6, *p < 0.05), (e) Zonula Occludens-1 (n = 5–6, *p < 0.05), (f) Occludin (n = 5–6, *p < 0.05) and (g) Claudin-5 (Sham, day 1, day 3 n = 5–6, day 7 n = 3). (h) Immunohistochemistry analysis assessing the co-localization of the respective microvessel and pericyte markers, Lectin and PDGFRβ. Scale bar: 20 μm. Data expressed as mean ± S.E.M. One way ANOVA with Dunnett post hoc test