Fig. 4

Cyclosporine A (CsA) treatment improves BBB stabilization and closure in wildtype mice. Wildtype mice were subject to TBI before administration of 20 mg/kg CsA or vehicle at 15 min post-injury. (a) Evans blue analysis of BBB permeability in the ipsilateral cortex analyzed at 1 and 5 days post-injury (n = 5 per group and timepoint, ***p < 0.001, **p < 0.05). Isolated microvessels analyzed by RT-QPCR for (b) Mmp-9 (n = 4, ***p < 0.001, **p < 0.01 vs treatment sham, ⁺⁺p < 0.01 vs relevant timepoint), (c) Pdgfrβ (n = 4, ***p < 0.001) (d) Zonula Occludens-1 (n = 4, ***p < 0.001, *p < 0.05 vs treatment sham, ⁺⁺p < 0.01 vs relevant timepoint) and (e) Occludin (n = 4, ***p < 0.001, *p < 0.05) mRNA expression. All data expressed as mean ± S.E.M. Two-way ANOVA with Bonferroni post hoc test