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Fig. 1 | Molecular Neurodegeneration

Fig. 1

From: Loss of XBP1 accelerates age-related decline in retinal function and neurodegeneration

Fig. 1

Reverse-transcription quantitative PCR reveals aged mice have a compromised ER stress response. a Reverse-transcription quantitative PCR (qPCR) was performed on total RNA extracted from whole retina from 4 month old (n = 4) and 20–24 month (n = 3) old XBP1 fl/fl (WT) mice. Primers specific for spliced Xbp1 (Xbp1s) reveal a 50% reduction in mRNA level in aged retina. Conversely, CHOP mRNA is significantly elevated in aged retina compared to young adult. b Graph of relative mRNA expression of XBP1s using RNA extracted from WT retinal explants subjected to vehicle or 5 μm thapsigargin (TG) for 6 h ex vivo. Retina from one month old mice (n = 3) respond to TG with a significant 2-fold increase in XBP1s. In contrast, retina from 13 month old mice (n = 3) have a minor 18% increase in XBP1s expression. All qPCR data normalized to 18 s. *, p < 0.02; **, p < 0.001

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