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Fig. 4 | Molecular Neurodegeneration

Fig. 4

From: Loss of XBP1 accelerates age-related decline in retinal function and neurodegeneration

Fig. 4

Retinal degeneration evident at 12–14 months of age in XBP1 fl/fl; Chx10-cre compared to wild type. a DAPI stained cryosections of XBP1 fl/fl (WT) and XBP1 fl/fl; Chx10-Cre (cKO) retina at P15 and 12 months, and a WT cryosection at 24 months, that closely represent the average retinal layers for each genotype and age. b Graph of measurements of retinal layers showing statistically significant differences in the outer nuclear layer (ONL, p < 0.02) and inner plexiform layer (IPL, p < 0.001) between WT (n = 7) and XBP1 cKO (n = 5) at 12–14 months (13 mo.). Graph also depicts statistically significant differences in the outer nuclear layer (ONL, p < 0.01) and inner plexiform layer (IPL, p < 0.01) between WT at 12–14 months and WT at 20–24 months (22 mo., n = 4). c Antibodies against Brn3a (red, arrowhead) label a majority of RGCs in WT (upper left) and XBP1 cKO (upper right) at 12–14 months. Sections are counterstained with DAPI (blue). Low magnification of a cryosection through the optic disk (OD, bottom). d Graphs show the number of Brn3a-positive cells within the ganglion cell layer at P15 (data from Fig. 1h), 13 months, and at 22 months. There is a statistically significant decrease (p < 0.01) in Brn3a-positive cells at age 13 months between WT (black, n = 6) and XBP1 cKO (grey, n = 5). In addition, there is a statistically significant decrease (p < 0.01) in Brn3a-positive cells between WT at age 13 months and at age 22 months (n = 4). INL, inner nuclear layer. Scale bar = 40 μm in A and 60 μm in C, upper, and 350 μm in C, lower

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