Fig. 7
From: Loss of XBP1 accelerates age-related decline in retinal function and neurodegeneration
Glycolysis is less efficient in XBP1 fl/fl; Chx10-Cre retina than in age-matched wild type retina. a Graph depicting the normalized extracellular acidification rate (ECAR) at twelve measurement points for WT (black, n = 5 retinas, 18 explants) and XBP1 fl/fl; Chx10-Cre (cKO, grey, n = 5 retinas, 18 explants) retinal explants, aged 12–15 months throughout a glycolysis stress test in a Seahorse extracellular flux analyzer. Injections of glucose, oligomycin, and 2-D-glucose (2-DG) were made as indicated. b Baseline ECAR is significantly (p < 0.01) reduced in XBP1 cKO compared to WT, as is peak glycolysis (p < 0.04). A mitochondrial stress test reveals no differences in oxygen consumption rate (OCR) between retinal explants from 12 to 15 month old WT (n = 4 retinas, 18 explants) and XBP1 cKO (n = 4 retinas, 18 explants) mice