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Table 1 Antibodies used in immunofluorescence (IF) and western blot analyses (WB)

From: Loss of XBP1 accelerates age-related decline in retinal function and neurodegeneration

Antibody

Dilutions

Catalog No.

Company

anti-Ribeye, B-domain

1:800 (IF)

192,003

Synaptic Systems

anti-Pkc-α

1:400 (IF)

sc-8393

Santa Cruz Biotechnology

anti-Calretinin, clone 6B8.2

1:800 (IF)

Mab1568

Millipore

anti-Iba1

1:800 (IF)

019–19,741

Wako

anti-XBP1

1:1000 (WB)

sc-7160

Santa Cruz Biotechnology

anti-Glutamine synthetase

1:800 (IF)

Mab302

Millipore

anti-Brn3a

1:800 (IF)

sc-31,984

Santa Cruz Biotechnology

anti-Pax6

1:40 (IF)

Pax6-s

DSHB

anti-β actin

1:10,000 (WB)

ab8226

Abcam

Peroxidase

1:10,000 (WB)

PI-2000

Vector

Peroxidase

1:10,000 (WB)

PI-1000

Vector

Texas Red

1:800 (IF)

T6391

Molecular Probes

Alexa Fluor-488

1:800 (IF)

A11001

Molecular Probes

Alexa Fluor-594

1:800 (IF)

A11005

Molecular Probes

Alexa Fluor-594

1:800 (IF)

A11080

Molecular Probes

anti-Calbindin D-28 K, CB-955-955

1:100 (IF)

Sab4200543

Sigma