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Fig. 4 | Molecular Neurodegeneration

Fig. 4

From: Differential induction of mutant SOD1 misfolding and aggregation by tau and α-synuclein pathology

Fig. 4

G85R-SOD1:YFP does not form inclusions in the forebrain of rTg4510/G85R-SOD1:YFP bigenic. The severity of neurofibrillary tangle pathology in the hippocampus and cortex (a, cortex shown immunostained with the MC1 antibody, red) in rTg4510 mice is similar to that of rTg4510/G85R-SOD1:YFP bigenic mice (b). Although the intensity of YFP fluorescence in the trigenic P301L/G85R-SOD1:YFP mice was higher than that of mice expressing G85R-SOD1:YFP alone, but there was no evidence of organization into inclusion structures (c and d; see Additional file 6: Figure S6). There were isolated cells that were hyperfluorescent (d), but the fluorescence in these cells did not appear to be organized into fibrils. All images were taken at 60X magnification of 5 μm paraffin-embedded sections. Nuclei were stained with DAPI (blue). Exposure time and specifications were kept consistent across all images, optimized to the rTg4510/G85R-SOD1:YFP tissue sections. Representative images are shown for 5 rTg4510/G85R-SOD1:YFP mice (1 male, 4 female) and 4 G85R-SOD1:YFP mice (4 males) between 8 and 9 months of age

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