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Fig. 1 | Molecular Neurodegeneration

Fig. 1

From: Tolerogenic bone marrow-derived dendritic cells induce neuroprotective regulatory T cells in a model of Parkinson’s disease

Fig. 1

Surface expression on BMDCs. GM-CSF-generated BMDCs were cultured in media alone or with 20 ng/ml GM-CSF for 2 days prior to stimulation with 30 μg/ml N-α-Syn for 1 day. Treatment groups were as follows: (1) media-cultured, unstimulated BMDCs; (2) GM-CSF-cultured, unstimulated BMDCs; (3) media-cultured, N-α-Syn-stimulated BMDCs; and (4) GM-CSF-cultured, N-α-Syn-stimulated BMDCs. Cells were harvested and reacted with antibodies to detect expression of CD11c, CD11b, MHC II, CD86, OX40L, Jag-1, CD39 and CD73, then evaluated by flow cytometric analysis. a Cells were gated by forward scatter area vs height to include only single cells and  CD11b+CD11c+ BMDC populations were identified. Percentages of cells expressing CD11b or CD11c were determined and the mean percentages of single cells positive for each marker are shown within the bars. (b and c) BMDCs were gated to include the CD11b+CD11c+ cell population and the geometric mean fluorescent intensity (MFI) was determined for expression of MHC II, CD86, OX40L, Jag-1, CD39, and CD73. b Overlays of representative histograms are shown for BMDCs treated with media (red), GM-CSF (blue), N-α-Syn (orange), or GM-CSF + N-α-Syn (green). c Histograms represent the means ± SEM for 7 replicates from each treatment group. The means were compared by one-way ANOVA and Newman-Keuls post-hoc test whereby p ≤ .0.05 compared to BMDCs treated with amedia, bGM-CSF, or cN-α-Syn

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