Fig. 3

Cytokines and chemokines released from BMDCs. GM-CSF-generated BMDCs were pretreated with media or 20 ng/ml GM-CSF for 2 days and/or stimulated with 30 μg/ml N-α-Syn for 1 day. Treatment groups were as follows: (1) media-cultured, unstimulated BMDCs; (2) GM-CSF-cultured, unstimulated BMDCs; (3) media-cultured, N-α-Syn-stimulated BMDCs; and (4) GM-CSF-cultured, N-α-Syn-stimulated BMDCs. Supernatants were removed and assessed by Luminex xMAP proinflammatory cytokine and chemokine array. For each treatment condition,19–21 replicates were included in the array analyses. Means ± SEM of protein concentrations were assessed by one-way ANOVA and Tukey’s post-hoc test whereby p ≤ .0.05 compared to BMDCs treated with ^amedia, ^bGM-CSF, or ^cN-α-Syn