Fig. 7

Gene expression in midbrain after treatment with MPTP or BMDCs and MPTP. BMDCs were differentiated for 8 days in 20 ng/ml GM-CSF prior to 3 days of pretreatment in media alone. These BMDCs were transferred i.v. one and two weeks prior to intoxication with four doses of 16 mg/kg MPTP. Two days after intoxication, PBS, MPTP and BMDC + MPTP mice were sacrificed and the brain was removed, hemisected and the midbrain was incubated in RNAlater for 24 h prior to freezing at − 80 °C. RNA was isolated from the midbrain, converted to cDNA PCR arrays of proinflammatory genes were run. a Gene expression was determined relative to the PBS control midbrains; n = 3 for PBS and n = 4 for MPTP and MPTP+BMDCs. Fold change was determined using SA Bioscience software. b Ingenuity Pathway Analysis was used to determine the expression of genes associated with the inflammatory response in BMDCs + MPTP midbrain compared to MPTP control mice