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Fig. 1 | Molecular Neurodegeneration

Fig. 1

From: Inoculation of α-synuclein preformed fibrils into the mouse gastrointestinal tract induces Lewy body-like aggregates in the brainstem via the vagus nerve

Fig. 1

Inoculation of α-synuclein (α-Syn) preformed fibrils (PFFs) into the mouse gastric wall. a Coomassie brilliant blue (CBB) staining and western blot (WB) analysis of α-Syn. Smear bands are observed in PFFs. b Thioflavin T assay. High-intensity fluorescence at 535 nm is detected in PFFs (n = 3). A. U., arbitrary unit. c Transmission electron microscopy of α-Syn PFFs. Scale bar 200 nm. d-h α-Syn immunohistochemistry in the stomach at 45 days postinoculation. d α-Syn deposition in the submucosa (arrowheads). Scale bar 100 μm. e High-magnification image of α-Syn deposition in the submucosa. Scale bar 20 μm. f High-magnification image of neurons in the myenteric plexus. Scale bar 20 μm. g α-Syn deposition in the muscular layers. Neurons in the myenteric plexus (arrow). Scale bar 100 μm. h High-magnification image of α-Syn deposition in the muscular layers. Scale bar 20 μm. Data are the mean ± SEM

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Molecular Neurodegeneration

ISSN: 1750-1326