Fig. 7

A small molecule enhancer of miRNA biogenesis stimulates paraspeckle assembly in neuroblastoma cells. a and b SH-SY5Y cells were treated with enoxacin, riluzole, edaravone and HDAC inhibitors trichostatin A (TSA) and sodium butyrate (NaB), and paraspeckle assembly was assessed by NEAT1_2 RNA-FISH (a) and qRT-PCR (n = 4–6, b). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Kruskal-Wallis test with Dunn’s correction for multiple comparisons). Cells were harvested for analysis after 4 h of TSA and NaB treatment (500 nM and 2 mM, respectively) and after 24 h of enoxacin, riluzole and edaravone treatment (all 10 μM). Scale bar, 10 μm