Table 1 Primers used in the study
Target | Forward | Reverse |
|---|---|---|
GAPDH | 5’-TCGCCAGCCGAGCCA-3′ | 5’-GAGTTAAAAGCAGCCCTGGTG − 3′ |
NEAT1 total | 5’-CTCACAGGCAGGGGAAATGT-3′ | 5’-AACACCCACACCCCAAACAA-3′ |
NEAT1_2 | 5’-AGAGGCTCAGAGAGGACTGTAACCTG-3′ | 5′-TGTGTGTGTAAAAGAGAGAAGTTGTGG-3’ |
TDP-43 | 5’-TCAGGGCCTTTGCCTTTGTT-3’ | 5’-TGCTTAGGTTCGGCATTGGAT-3’ |
IL8 | 5’-ACACTGCGCCAACACAGAAA-3′ | 5’-CCTCTGCACCCAGTTTTCCT-3’ |
ADARB2 | ATATTCGTGCGGTTAAAAGAAGGTG | ATCTCGTAGGGAGAGTGGAGTCTTG |
Alu RNA | 5’-GAGGCTGAGGCAGGAGAATCG-3’ | 5’-GTCGCCCAGGCTGGAGTG-3’ |
DICER | 5’-TTAACCTTTTGGTGTTTGATGAGTGT-3’ | 5’-GCGAGGACATGATGGACAATT-3’ |
DROSHA | 5’-CGGCCCGAGAGCCTTTTAT-3’ | 5’-TGCACACGTCTAACTCTTCCA-3’ |
ADAR1 | 5’-TTGTCAACCACCCCAAGGT-3’ | 5’-CCATCAGCCAGACACCAGTT-3’ |
AGO2 | 5’-CACCATGTACTCGGGAGCC-3’ | 5’-TCCCAAAGTCGGGTCTAGGT-3’ |
FUS | 5’-GCGGGGCTGCTCAGT-3’ | 5’-TTGGGTTGCTTGTTGGGTAT-3’ |
CHOP | 5’-TTAAAGATGAGCGGGTGGC-3′ | 5’-GCTTTCAGGTGTGGTGATGTA-3’ |
CXCL10 | 5’-TGCCATTCTGATTTGCTGCC-3’ | 5’-ATGCTGATGCAGGTACAGCG-3’ |
IFNB1 | 5’-ACGCCGCATTGACCATCTAT-3’ | 5’-AGCCAGGAGGTTCTCAACAA-3’ |
IFNA1 | 5’-TCTGCTATGACCATGACACGAT-3’ | 5’-CAGCATGGTCCTCTGTAAGGG-3’ |
IFNA2 | 5’-AGGAGGAAGGAATAACATCTGGTC-3’ | 5’-GCAGGGGTGAGAGTCTTTGAA-3’ |
MALAT1 | 5’-GGATCCTAGACCAGCATGCC-3’ | 5′- AAAGGTTACCATAAGTAAGTTCCAGAAAA-3’ |
IFIH1 | 5’-GCATGGAGGAGGAACTGTTGA-3’ | 5’-GCATGGAGGAGGAACTGTTGA-3’ |
CYCS | 5’-TCGTTGTGCCAGCGACTAAA-3’ | 5’-GCTTGCCTCCCTTTTCAACG-3’ |
STAT1 | 5’-CTGTGCGTAGCTGCTCCTTT-3’ | 5’-GGTGAACCTGCTCCAGGAAT-3’ |
MYD88 | 5’-TGACCCCCTGGGGCAT-3’ | 5’-AGTTGCCGGATCATCTCCTG-3’ |
Pri-miR-17–92 | 5’-CAGTAAAGGTAAGGAGAGCTC AATCTG-3’ | 5’-CATACAACCACTAAGCTAAAGAAT AATCTGA-3’ |
Pri-miR-15a | 5’-CCTTGGAGTAAAGTAGCAGCAC-3’ | 5’-CCTTGTATTTTTGAGGCAGCAC-3’ |
miR-18a | 5’-CATCATCGGTAAGGTGCATC-3’ | 5’-GAATCGAGCACCAGTTACGC-3′ (unimiR) |
miR-92a | 5’-GAGTCTATTGCACTTGTCCC-3’ | unimiR |
miR-106a | 5’-AAAAGTGCTTACAGTGCAGGTAG-3’ | unimiR |