Fig. 5

Modest concordance between transcriptomic and proteomic changes in microglia in acute neuroinflammatory and neurodegenerative disease conditions. a Scatter plot showing level of concordance between differentially expressed proteins and differentially expressed gene transcripts comparing LPS-treated WT to untreated WT microglia. The current microglial dataset was compared to transcriptomic data for shared gene symbols in a previously published RNA sequencing study of acutely isolated microglia from WT and LPS-treated WT mice [25]. Only differentially expressed proteins/transcripts in both datasets are shown. Gene symbols in the top right or bottom left quadrants indicate concordant changes. b Scatter plot showing level of concordance between differentially expressed proteins and differentially expressed gene transcripts comparing 5xFAD to WT microglia [24]. Our microglial proteomic dataset was cross referenced with an existing microarray gene expression study of acutely isolated WT and 5xFAD mouse microglia. Concordant changes are indicated in the top right and bottom left quadrants. c Heat map representing expression changes in 5xFAD microglia (vs. WT microglia) seen only at the protein level (left) or at the transcriptomic level (right)