Fig. 11
The distal degeneration of forelimbs is slower than that of hindlimbs in SOD1 mutant mice. a-c Confocal micrographs of longitudinal sections of the sciatic nerve and radial nerves of G93A+/+ mice at 140 d showing high expression of MHCI, GFAP and p75NTR in the sciatic nerves but not in radial nerves. Bar, 100 μm. d Percentages of muscle atrophy (relative to NTG mice) of TB, TA, and GC muscles of G93A+/+ mice at 140 d. Data are expressed as mean ± SEM. Ten, 16 and ten independent experiments were analyzed for TB, TA and GC, respectively. Data are reported as mean ± SEM. *P < 0.05; **P < 0.01; (TA; GC vs TB) by one-way ANOVA with Tukey’s post analysis. e-g Quantification of (e) MHCI, (f) GFAP and (g) p75NTR immunoreactivity in sciatic (SN) and radial (RN) nerves of G93A+/+ mice compared to NTG littermates at 140 d. The analysis was done on radial and sciatic nerves of the same animals. Data are expressed as mean ± SEM from three independent experiments (at least four serial sections for each animal) for each genotype. ***P < 0.001; ****P < 0.0001 (G93A_RN vs G93A_SN) °°P < 0.01; °°°P < 0.001; °°°°P < 0.0001 (G93A_SN or G93A_RN vs NTG_SN or NTG_RN) by one-way ANOVA with Tukey’s post analysis