Fig. 4From: Counteracting roles of MHCI and CD8+ T cells in the peripheral and central nervous system of ALS SOD1G93A miceMHCI depletion reduces the inflammation in the lumbar and cervical spinal cord of mSOD1 mice during the disease course. a, b DAB immunostaining for CD68 in the lumbar spinal cord of NTG; G93A+/+ and G93A−/− mice at 123 and 140 d. Bar, 50 μm. c Immunofluorescence staining for MHCI (purple) and motor neurons (neuro-trance, NT; blue) in the cervical spinal cord of NTG; G93A+/+ and G93A−/− mice at 140 d. The inset shows a magnification of MHCI-labeled microglia surrounding MNs. The images are representative of at least four sections from two independent experiments from each genotype. Bar, 50 μm; Inset Bar: 50 μm. d Immunofluorescence staining for CD68 in the cervical spinal cord of NTG; G93A+/+ and G93A−/− mice at 140 d. Bar, 50 μm (e, i) Quantification of CD68 staining in (e) lumbar and (i) cervical spinal cord hemisections of G93A+/+, G93A−/− mice compared to NTG+/+ littermates at 123 and 140 d. Data are expressed as mean ± SEM (four serial sections for each animal). At 123 d, four, six and four independent experiments were analyzed for NTG, G93A+/+ and, G93A−/− mice, respectively. At 140 d, in cervical and lumbar spinal cord, four independent experiments were analyzed for each genotype. f-h Real-time PCR for Ifn-γ, Ccl2 and Igf1 transcripts in the lumbar spinal cord of G93A+/+, G93A−/− mice compared to NTG+/+ littermates at 123 and 140 d. Data are normalized to β-actin and expressed as the mean ± SEM fold change ratio between G93A+/+ mice, G93A−/− mice and controls from four independent experiments for each genotype. j-l Real-time PCR for Ifn-γ, Ccl2 and Igf1 transcripts in the cervical spinal cord of G93A+/+, G93A−/− mice compared to NTG+/+ littermates at 140 d. Data are normalized to β-actin and expressed as the mean ± SEM fold change ratio between G93A+/+, G93A−/− and controls mice from four independent experiments for each genotype. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; (G93A+/+ vs G93A−/−); °P < 0.05; °°P < 0.01; °°°P < 0.001; °°°°P < 0.0001 (G93A−/−; G93A+/+ vs NTG) by one-way ANOVA with Tukey’s post-analysisBack to article page