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Fig. 8 | Molecular Neurodegeneration

Fig. 8

From: Counteracting roles of MHCI and CD8+ T cells in the peripheral and central nervous system of ALS SOD1G93A mice

Fig. 8

MHCI and CTLs depletion affect the motor axonal cytoskeleton in mSOD1 mice. a Representative immunoblot image of neurofilament (NF200) expression in sciatic nerve extracts from NTG, G93A+/+ and G93A−/− mice at 123 and 140 d. b Densitometric analysis indicated reductions in the expression levels of neurofilaments in the sciatic nerves of G93A−/− mice during disease progression. At each disease stage, data are reported as percentages of the relative NTG (mean ± SEM) from four independent experiments from each genotype. *P < 0.05; **P < 0.01 (G93A−/− vs G93A+/+); °°P < 0.01; °°°P < 0.001; (G93A−/−; G93A+/+ vs NTG+/+) by one-way ANOVA with Tukey’s post-analysis. c Confocal micrographs of transverse sections of the sciatic nerve of NTG+/+; NTG−/−,; G93A+/+ and G93A−/− mice, showing markedly lower expression of neurofilaments in G93A−/− mice. The images are representative of at least three sections from two independent experiments for each genotype. Bar, 30 μm. d Representative immunoblot images of tubulin-βIII and importin β in sciatic nerve extracts from NTG+/+, G93A+/+ and G93A−/− mice at 123 and 140 d. e, f Densitometric analysis indicated a reduction in the expression levels of (e) tubulin-βIII and (f) importin β in the sciatic nerves of G93A−/− during disease progression. At each disease stage, data are reported as the percentages of NTG (mean ± SEM) from four independent experiments from each genotype. *P < 0.05 **P < 0.01; ***P < 0.001 (G93A−/− vs G93A+/+); °P < 0.05; °°P < 0.001; °°°°P < 0.0001 (G93A−/−; G93A+/+ vs NTG) by one-way ANOVA with Tukey’s post-analysis

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