Fig. 9
MHCI and CTLs depletion affect the proliferation of Schwann cells in SOD1 mutant mice. a Representative immunoblot images of GFAP, phospho-ERK (P-ERK), and p75NTR in sciatic nerve extracts from NTG, G93A+/+ and G93A−/− mice at 123 and 140 d. b-d Densitometric analysis indicated a reduction in the expression levels of (b) GFAP and (c) P-ERK, but (d) and increased levels of p75NTR in the sciatic nerves of G93A−/− mice. Relative levels of P-ERK were normalized to levels of total ERK (not shown). At each disease stage, data are reported as the percentage of NTG (mean ± SEM) from four independent experiments from each genotype. *P < 0.05 ***P < 0.001; ****P < 0.0001 (G93A−/− vs G93A+/+); °P < 0.05; °° P < 0.01; °°°P < 0.001 (G93A−/−; G93A+/+ vs NTG) by one-way ANOVA with Tukey’s post-analysis. e-f Confocal micrographs of transverse sections of sciatic nerve of NTG+/+, NTG−/−; G93A+/+ and G93A−/− mice showing marked reduction in the expression of (e) GFAP and (f) vimentin in the PNS of NTG−/− and G93A−/− mice at 123 d; The images are representative of at least three sections from two independent experiments for each genotype. Bar, 30 μm