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Fig. 2 | Molecular Neurodegeneration

Fig. 2

From: Long-read sequencing across the C9orf72 ‘GGGGCC’ repeat expansion: implications for clinical use and genetic discovery efforts in human disease

Fig. 2

Workflow for linearizing, pooling, and sequencing plasmids on the PacBio RS II and ONT MinION long-read platforms. Each plasmid was cut with the restriction enzyme identified in the respective plasmid maps (Fig. 1), and at the specified location. After linearizing each plasmid independently, the plasmids were pooled and cleaned. We then sequenced the same pool on the PacBio RS II and Oxford Nanopore Technologies’ (ONT) MinION using their respective library preparation protocols. After sequencing, reads from each plasmid were identified using BLAST and then aligned to their respective reference sequences using graphmap, as preparation for downstream comparisons

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