Transcriptional profiling of HERV-K(HML-2) in amyotrophic lateral sclerosis and potential implications for expression of HML-2 proteins

Table 2 Relative cloning frequencies and locus origins of cDNA sequences from an env gene 5′ region^*

locus designation	ALS				Ctrl			Type	Env	Rec/Np9	hg19
	motor	motor	motor	sc	motor	motor	sc
	89-Mot	62-Mot	64-Mot	802-SC	1509-Mot	73-Mot	4263-SC
chr1p31.1_K-1				4.00	7.69		4.00	1	606	Np9	chr1:75844857
chr1q23.3_K-18				4.00				1	553		chr1:160667241
chr3q12.3_K-5	62.50	77.50	70.00	28.00	53.85	40.00	40.00	1	263	Np9	chr3:101412824
chr3q13.2_K-3	6.25	2.50			7.69	6.67	4.00	1	434	Np9	chr3:112750203
chr3q21.2_K-4						13.33		2		Rec	chr3:125611223
chr5q33.3_K-10				8.00				1			chr5:156091809
chr11q23.3_K-20						6.67		1	431		chr11:118594283
chr19q11_K-19						6.67		2	699		chr19:28131235
Venter		7.50	10.00	28.00	15.38		20.00	1	452
chr1:155…/chr5:156…	31.25	12.50	20.00	24.00	15.38	13.33	32.00
chr8:80…/chr8:12…				4.00		13.33
# cDNA sequences	16	40	10	25	13	15	25

^*A total of 4 samples from ALS patients and 3 samples from controls were examined. A number of cDNA sequences could not be assigned with confidence to either one of two different HML-2 loci on chr1:155,598,877–155,599,165 or chr5:156,087,137–156,087,425, and chr8:8,061,161–8,061,741 or locus chr8:12,318,971–12,319,551. Note that the skew towards type 1 loci is caused by lower amounts of RT-PCR product from type 2 loci and therefore relatively few clones with type 2-derived PCR-product (see the main paper text). See also legends to Tables 1 and 2 for more details

ISSN: 1750-1326