Fig. 3
Administration of anti-TLR2 (T2.5) decreases α-synuclein pathology in a synucleinopathy mouse model. a Experimental scheme. Non-tg and α-Syn-tg (Line 61) mice were administrated with either IgG (5 mg/kg) or T2.5 (5 mg/kg) weekly for 4 weeks. The levels of α-synuclein pathology, glial cell reactivity, neurodegeneration, and behavioral deficits were analyzed after a 5-weeks post injection. b Representative images from immunohistochemical staining of α-synuclein in the neocortex and hippocampus of mice. The level of α-synuclein was analyzed by optical density quantification (n = 6 per group). c Representative images from immunohistochemical staining of PK-resistant α-synuclein in the neocortex and hippocampus of mice. The levels of PK-resistant α-synuclein were analyzed by optical density quantification (n = 6 per group). d Immunoblot analysis of mice brain lysates. Triton-soluble and -insoluble brain lysates were probed for α-synuclein and β-actin. The levels of triton-insoluble α-synuclein monomer and high molecular weight oligomers were determined by densitometric quantification (n = 4 per group). Data are mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001, n.d. not detected; one way ANOVA for (b, d) and unpaired t test for (c). Scale bars, 250 μm (low magnification) and 25 μm (high magnification)