Fig. 5

Neuroprotective effect of anti-TLR2 (T2.5) treatment in synucleinopathy mouse model. Non-tg or α-Syn-tg (Line 61) mice were administrated with either IgG (5 mg/kg) or T2.5 (5 mg/kg) weekly for 4 weeks. a Representative images from immunohistochemical staining of NeuN in the neocortex and hippocampus of mice. The numbers of NeuN positive cells were counted in the neocortex and hippocampus of mice (n = 6 per group). b Representative images from immunohistochemical staining of Thyroxine hydroxylase (TH) in the antibody-administrated mice. The level of TH was analyzed by optical density quantification and the numbers of TH positive cell were counted in the striatum and substantia nigra of mice, respectively (n = 6 per group). c Immunoblotting analysis of whole-brain lysates. The lysates were probed for active form of casepase-3 and β-actin. The level of active caspase 3 was determined by densitometric quantification (n = 3 per group). d–f Behavioral analysis of the mice. The total activity (d), latency (e), and thigmotaxis (f) were analyzed by open field test (n = 6 per group). Data are mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001; one way ANOVA. Scale bar, 25 μm