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Fig. 1 | Molecular Neurodegeneration

Fig. 1

From: PU.1 regulates Alzheimer’s disease-associated genes in primary human microglia

Fig. 1

Characterisation of culture conditions for microarray analysis. a Characterisation of primary human mixed glial cultures comprised of pericytes (PDGFRβ), microglia (PU.1), astrocytes (GFAP), and endothelial cells (PECAM1), compared with pericyte-only cultures containing PDGFRβ only, scale bar = 100 μm. b PU.1 knockdown in mixed glial cultures transfected with 50 nM scrambled or PU.1 siRNA for 7 days, scale bar = 100 μm. c Microarray analysis of cell specific genes in scrambled siRNA transfected mixed glial cultures or pericyte-only cultures. d Unbiased hierarchical clustering of the top 250 differentially expressed genes from microarray analysis of mixed glial cultures or pericyte cultures transfected with 50 nM scrambled or PU.1 siRNA for 7 days, n = 3 independent microglial cultures

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