Fig. 4
Post-treatment with local neutralisation of C1q and assessment of the retina at day 14 (a). b Post-treatment with anti-C1q monoclonal antibody (mAb), ANX-M1, immediately after 7 days of photo-oxidative damage (PD, day 7) reduced the number of TUNEL+ cells at 7 days of post damage (day 14), versus IgG control at day 14 (P < 0.05). c There was a significant reduction in photoreceptor loss in mice treated with ANX-M1 anti-C1q antibody (P < 0.05). d There was minimal effect on the IBA1+ microglia/macrophages in ANX-M1 anti-C1q antibody cohort compared to IgG control antibody (P > 0.05). e-g Post-treatment of retina with ANX-M1 anti-C1q antibody displayed a larger a-wave (e) and b-wave (f) compared to IgG control cohort, which was most pronounced at 1.9 log cd.s/m2 (g), indicating a significantly improved retinal function (P < 0.05). h Cone response demonstrated a significant improvement in ANX-M1 anti-C1q antibody cohort, compared to IgG control (P < 0.05). Statistical analyses were determined by an unpaired student t-test or two-way ANOVA followed by post-hoc multiple comparison (* denotes P < 0.05; NS denotes no significance)