Table 1 Primary antibodies used for immunohistochemistry and Western blot
Antibody | Target | Dilution | Source | Catalog # |
|---|---|---|---|---|
Rabbit α-IBA1 | Ionised calcium binding protein 1 | 1:500 | Wako Chemicals | 019-19,741 |
Goat α-IL-1β | IL-1 beta/IL1F2 | 1:1000 | R&D Systems | AF-501 |
Goat α-C3d | Complement component C3d | 1:2000 | R&D Systems | AF-2655 |
Rabbit α-C1q | C1q, clone 4.8 | 1:400 | Abcam | AB182451 |
Rabbit α-C1q | C1q complement isolated from human plasma | 1:1000 | Dako | F0254 |
Rabbit α-NALP3/NLRP3 | 1-50 amino acids of human NLRP3 | 1:500 | Novus Biologicals | NPB2-12446SS |
Rat α-F4/80 | F4/80 clone A3-1 | 1:100 | Abcam | AB6640 |
Rabbit α-GAPDH | GAPDH | 1:4000 | Sigma Aldrich | G9545 |