Fig. 4

Detection of C-terminal and N-terminal fragments of Ng in digests by calpain. Digestion products were analyzed in reducing SDS PAGE gels (12%). Panel a Coomassie stain followed by silver stain; Panel b NG36 immunoblot; Panel c, H-6 immunoblot. Lanes 1, Ng control; lanes 2, 3 and 4, Ng digest with 90-, 30-, 10-fold diluted calpain-1, respectively. The location of the parent Ng1–78 band is indicated on the right near panel c with an arrow, those of the major ~ 5 kDa C-terminal fragments by an * in panel b, and those of the main N-terminal fragment near 6.5 kDa by a # in panel c. Size markers for panel a as indicated