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Fig. 9 | Molecular Neurodegeneration

Fig. 9

From: Alzheimer-associated cerebrospinal fluid fragments of neurogranin are generated by Calpain-1 and prolyl endopeptidase

Fig. 9

Active fractions during enzyme enrichment show cleaving activity at Ng75_76 (MALDI-TOF analysis). Synthetic KKK-Ng50–78 was incubated with an active fraction and the reaction mix separated by HPLC. MALDI-TOF analysis of one of the HPLC fractions showed the presence of an [M + H]+ peak at 2570.3 Da which is consistent with a cleavage at Ng75_76 which releases –SGD (mass difference of 259 Da relative to the substrate KKK-Ng50–78)

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Molecular Neurodegeneration

ISSN: 1750-1326