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Table 1 Primers and PCR conditions used for detecting splice pattern (Forward: Fwd; Reverse: Rev)

From: The Trem2 R47H Alzheimer’s risk variant impairs splicing and reduces Trem2 mRNA and protein in mice but not in humans

Name

Sequence

Reaction condition

MmTrem2 Fwd

GCTCAATCCAGGAGCACAGT

95 °C 1 min; (95 °C 30s, 65 °C 15 s, 72 °C 1 min) *35 cycle; 72 °C 10 min

MmTrem2 Rev

TCTGACACTGGTAGAGGCCC

HsTREM2 Fwd

GCCTGACATGCCTGATCCTC

95 °C 1 min; (95 °C 30s, 65 °C 15 s, 72 °C 1 min) *35 cycle; 72 °C 10 min

HsTREM2 Rev

AGGACCTTCCTGAGGGTGTC

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Molecular Neurodegeneration

ISSN: 1750-1326