Fig. 2

LRP1-dependent Cy3-Aβ42 uptake by pericytes in mouse brain slices. a A diagram illustrating the experimental procedure in cultured mouse brain slices used to determine Cy3-Aβ42 uptake by pericytes. Brain slices were first cultured in transwell inserts with oxygenated aCSF (see method) for 4 h before adding Cy3-Aβ42 (1 μM). b-c Representative low-magnification images (b) and quantification (c) of cellular uptake of Cy3-Aβ42 by CD13+ pericytes in brain slices at 30 min and 2 h after the addition of Cy3-Aβ42. Scale bar: 25 μm. d Representative high magnification images showing Cy3-Aβ42 internalization by CD13-positive pericytes in brain slices in 2 h, in the presence of NI-IgG, anti-LRP1 or RAP. Scale bar: 20 μm. Orthogonal views on the right show Cy3-Aβ42 accumulation in CD13+ pericytes; scale bar: 5 μm. e Quantification of Cy3-Aβ42 uptake by CD13+ pericytes in mouse brain slices with and without NI-IgG, anti-LRP1, and RAP. N = 4 independent cultures; mean ± s.e.m.; p < 0.05 by One-way ANOVA followed by Bonferroni post-hoc test. Asterisks show colocalization of Cy3-Aβ42 and CD13 signals in (b) and (d)