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Table 1 Primary antibody information

From: GSK3β-mediated tau hyperphosphorylation triggers diabetic retinal neurodegeneration by disrupting synaptic and mitochondrial functions

Antibody Host Application Source Cat. No
β-tubulin Rabbit WB(1:1000) CSTa #2146
Ac-Tubulin Rabbit WB(1:1000)/IHC(1:100) CST #3971
Akt(pan) Rabbit WB(1:1000) CST #4691
pS473-Akt Rabbit WB(1:1000) CST #4060
Cleaved caspase-3 Rabbit WB(1:1000) CST #9664
Complex I Rabbit WB(1:1000)/IHC(1:100) Abclonal A7862
Complex IV Rabbit WB(1:1000) Abclonal A6564
GAPDH Mouse WB(1:3000) Abclonal AC033
GSK3β Rabbit WB(1:1000) CST #9315
pS9-GSK3β Rabbit WB(1:1000) CST #9322
IRS1 Rabbit WB(1:1000) CST #2382
MAP2 Chicken IHC(1:10000) Abcam ab75713
Synaptophysin Rabbit WB(1:5000)/IHC(1:100) Abcam ab32127
Tau-1 Mouse WB(1:5000)/IHC(1:100) EMB Millipore MAB3420
Tau-5 Mouse/
Rabbit
WB(1:5000)/IHC(1:100) Abcam/
Beyotime
Ab80579/
AF1249
pT205-Tau Rabbit WB(1:1000)/IHC(1:100) Abclonal AP0168
pT231-Tau Rabbit/
Mouse
WB(1:1000)/IHC(1:100) Abclonal/
Abcam
AP0401/
ab89748
pS396-Tau Rabbit WB(1:1000)/IHC(1:100) Abclonal AP0163
pS404-Tau Rabbit WB(1:1000)/IHC(1:100) Abcam ab92676
Thy1 Mouse IHC(1:100) Abcam ab225
TUJ1 Rabbit IHC(1:100) Abcam Ab18207
Tyr-tubulin Mouse IHC(1:100) Sigma-Aldrich T9028
  1. aCST Cell Signaling Technology