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Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: Genome-wide RNAseq study of the molecular mechanisms underlying microglia activation in response to pathological tau perturbation in the rTg4510 tau transgenic animal model

Fig. 3

Identification and validation of differential expression genes (DEGs). a. Volcano plot of DEGs in rTg4510 transgenic microglia relative to WT microglia at indicated age. Fold change are plotted against the –log(p value). The vertical empty space indicates the 1.5 fold change cutoff threshold. b. Validation of selected DEGs by q-RT-PCR. Twenty eight DEGs, 22 up-regulated and 6 down-regulated ones, were selected for q-RT-PCR. Log (fold change, rTg4510 vs. WT microglia RNA) of q-RT-PCR results of the original RNA samples (circles) and an independent set of RNA samples (squares) are plotted against RNAseq results (triangulars). Genes are ordered from left to right based on highest to lowest fold change values of RNAseq results. c. Venn diagram of the number of DEGs in the three studies as labelled. The number of common DEGs is shown in the overlapping areas. Enriched KEGG pathways are listed on bottom. d. Heat map of genes common to the three studies. The color intensity represents the log2 fold change of the expression

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