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Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: Inhibition of monocyte-like cell extravasation protects from neurodegeneration in DBA/2J glaucoma

Fig. 3

The majority of monocytes entering ONH tissue during glaucomatous progression are platelet bound. We have previously demonstrated that monocytes entering the ONH tissue during glaucoma are CD45hi/CD11b+/CD11c+ (Additional file 1: Figure S1 and [9]). RNA-sequencing analysis of ONH monocytes implicates platelets early in glaucoma pathogenesis. To determine if monocyte-platelet aggregates were present in the ONH in glaucoma flow cytometry was performed. a and b Antibodies against CD41 (a platelet marker) demonstrated that the majority (> 90%) of CD45hi/CD11b+/CD11c+ ONH monocytes were also platelet bound (n = 18 ONHs) compared to < 2% of PBMCs (n = 10 peripheral blood samples). c When visualized using imaging flow cytometry there were evident platelets bound to monocytes in the ONH (green, c) (n = 4 ONHs). Examples of monocyte-platelet aggregates and platelet negative monocytes are shown. BV = brilliant violet (blue/violet), FITC = fluorescein isothiocyanate (green), PE = phycoerythrin (yellow/orange), APC = allophycocyanin (red)

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