Fig. 6
From: Inhibition of monocyte-like cell extravasation protects from neurodegeneration in DBA/2J glaucoma
Genetic ablation of CD11b (Itgam) decreases monocyte number in the ONH. To determine whether genetic ablation of Itgam affects monocyte entry into the ONH during glaucoma, D2.Itgam−/− mice and genetic controls were harvested at 10.5 mo and ONHs assessed for monocytes by flow cytometry. a CD45+ and CD45hi cell number in the ONH was significantly decreased in Itgam−/− mice (a, left). Homozygous knockout mice lack CD11b and so the gating strategy compared to % viable cells. The relative proportions of CD45hi and CD45hi/CD11c+ types of myeloid derived cells were not changed (a, right) (n = 12 (D2.Itgam+/+), 10 (D2.Itgam−/−)). b Example flow plots and gating strategy