Fig. 3
From: Glucose transporter 1 critically controls microglial activation through facilitating glycolysis
Changes in glucose uptake and GLUT expression during microglial activation. BV2 and B6M7 cells were untreated or treated with LPS + IFNγ or IL-4 for 24 h. Cells were then processed for real-time RT-PCR (a, c), glucose uptake (b) or Western blot (d). a The expression of inflammatory mediators in naïve, LPS + IFNγ or IL-4 treated BV2 and B6M7 cells. N = 3, *, P < 0.05; **, P < 0.01, ***P < 0.001. b Glucose uptake in non-treated control, LPS + IFNγ or IL-4 treated BV2 and B6M7 cells. N = 3, *, P < 0.05. c mRNA expression of different GLUTs in control, LPS + IFNγ or IL-4 treated B6M7 cells. N = 3, ***, P < 0.001 compared to naïve microglia. d Western blot showing GLUT1, 4, 5, 9, 10, 12 in control (naïve), LPS + IFNγ treated, and IL-4 treated B6M7 cells. β-actin was used as housekeeping control. e Densitometric analysis showing GLUT expression related to control in LPS + IFNγ or IL-4 treated B6M7 cells. N = 3, ***, P < 0.001 compared to naïve microglia